<?xml version="1.0" encoding="UTF-8"?><rss version="2.0"><channel><title><![CDATA[Bioflux - Aquaculture, Aquarium, Conservation & Legislation]]></title><description><![CDATA[Articles]]></description><link>http://www.bioflux.com.ro/</link><copyright><![CDATA[Copyright Bioflux - Aquaculture, Aquarium, Conservation & Legislation]]></copyright><generator>sNews CMS</generator><item><title><![CDATA[Study of HSP genes in three highly economical valuable fish species in view to their improvement]]></title><description><![CDATA[    Funding Programe: The National Plan II of Reserch, Development and Innovation    

  Research Project PN II, no 51-056.   Partnerships in Prioritary Fields  .  

    Funding Source:    

    National Authority for Scientific Research (Romania)    

    Monitored by:    

    National Center&nbsp;for Management of&nbsp;Programs     

    Coordinator Institution:    

    University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca  &nbsp;  

    Partners:    

    Babes-Bolyai University, Cluj-Napoca    

    Alexandru Ioan Cuza University, Iasi    

    Budget/project:    

  1865196 lei  

    Period:    

  18, september 2007 – 17, september 2010  

    The Project's Team -&nbsp;    USAMV Cluj:    

  Prof. Dr. Hab.&nbsp;Ioan Bud  

  CS II. Dr.&nbsp;Ioan Valentin Petrescu-Mag  

  Prof. Dr. Hab.&nbsp;Doru Pamfil  

  Asist. Prof.&nbsp;Anca Boaru  

  Assoc. Prof. Dr. Viorica Cosier  

  Prof. Dr. Hab. Augustin Vlaic  

  Assoc. Prof. Dr. Dana Pusta  

  Assoc. Prof. Dr. Daniela Ladosi  

  Asist. Prof. Vlad Viorel Vladau  

  PhD stud. Sorin Nicolae&nbsp;Pop  

  Res. Asist. Cristian Sisea  

  PhD stud. Miklos Botha  

  PhD stud. Valentin Balteanu  

  Asist. Prof. Dr. Rodica Pop  

  PhD stud. Reka G. Stefan  

  Lecturer Antonia Odagiu  

  Assoc. Prof. Dr. Crina Dacinia Petrescu  

  Econ. Adriana Husovschi  

  Stud. Radu Harsan  

  PhD. Stud.&nbsp;Daniel Cocan  

  PhD. Stud. Laszlo Csep  

  Stud. Ciprian Vanea  

  Tehn.support: Elisabeta Maxim  

    The Project's Team - UBB:    

  Prof. Dr. Hab. Octavian Popescu  

  Prof. Dr. Nicolae Dragos  

  Prof. Dr. Hab. Nicolae Coman  

  Prof. Dr. Manuela Dordea  

  Lecturer Dr. Anca Butiuc-Keul  

  Lecturer Dr. Ruxandra Malina Petrescu-Mag  

  CS III. Dr. Anette Damert  

  Asist. Prof. Dr. Iulia Lupan  

  CS III. Dr. Beatrice Simona Kelemen  

  PhD Stud. Beatrix-Katalin Ferencz  

  PhD Stud. Oana-Mihaela Herlea  

  PhD Stud. Ioan Bacila  

  PhD Stud. Anet-Monica Chilici  

  PhD Stud. Endre Jakab  

  PhD Stud. Dana Cristina Suteu  

  PhD Stud. Florentina Bianca Basangiu  

  PhD Stud. Szilard Lehel Bucs  

  PhD Stud. Csaba Istvan Nagy  

  PhD Stud. Miklos Balint  

  PhD Stud. Jozsef Szabolcs Demeter  

  PhD Stud. Sergiu Chira  

  PhD Stud. Kinga Melinda Csorba  

  PhD Stud. Bela Marosi&nbsp;  

  Econ.&nbsp;Floare Gal   

    The Project's Team -&nbsp;UAIC:    

  Lecturer Dr.&nbsp;Lucian Dragos Gorgan  

  Lecturer Dr. Mirela Mihaela Campeanu  

  Lecturer Dr. Victor Surugiu  

  Lecturer Dr. Stefan Zamfirescu  

  PhD.&nbsp;Stud.&nbsp;Laura Buburuzan  

  Master Stud. Sorina Raluca Gherasim  

  Stud. Alexandru Neagu  

    Abstract    

    Objectives    

    Dissemination:    

    ***    

    ***    

    

    
]]></description><pubDate>Thu, 11 Feb 2010 11:11:17 +0000</pubDate><link>http://www.bioflux.com.ro/PN2-51-056/study-of-hsp-genes-in-three-highly-economical-valuable-fish-species-in-view-to-their-improvement/</link><guid>http://www.bioflux.com.ro/PN2-51-056/study-of-hsp-genes-in-three-highly-economical-valuable-fish-species-in-view-to-their-improvement/</guid></item><item><title><![CDATA[Other Journals]]></title><description><![CDATA[    Advances in Agriculture &amp; Botanics - International Journal of the Bioflux Society   (Short title: AAB Bioflux)  


    Human &amp; Veterinary Medicine - International Journal of the Bioflux Society   (Short title: HVM Bioflux)  


    Advances in Environmental Sciences - International Journal of the Bioflux Society   (Short title: AES Bioflux)  


    Animal Biology &amp; Animal Husbandry - International Journal of the Bioflux Society   (Short title: ABAH Bioflux)  


    Extreme Life, Biospeology &amp; Astrobiology - International Journal of the Bioflux Society   (Short title: ELBA Bioflux)  


    Porcine Research   (Short title: Porc Res)  


    Rabbit Genetics   (Short title: Rabbit Gen)  


    Poeciliid Research   (Short title: Poec Res)  


    ProEnvironment Promediu    


    Ecoterra    

]]></description><pubDate>Sun, 08 Mar 2009 04:14:01 +0000</pubDate><link>http://www.bioflux.com.ro/other-journals/other-journals/</link><guid>http://www.bioflux.com.ro/other-journals/other-journals/</guid></item><item><title><![CDATA[The project results included in a book (in Romanian)]]></title><description><![CDATA[  Click here:  

      http://www.editura.bioflux.com.ro/docs/ceex140electro.pdf      
]]></description><pubDate>Sat, 01 Nov 2008 16:30:27 +0000</pubDate><link>http://www.bioflux.com.ro/ceex-140/the-project-results-included-in-a-book-in-romanian/</link><guid>http://www.bioflux.com.ro/ceex-140/the-project-results-included-in-a-book-in-romanian/</guid></item><item><title><![CDATA[Details regarding CEEX 140/2006]]></title><description><![CDATA[  Research Program: Research of Excelence  

  Financed by:   National Authority for Scientific Research    

  Monitor:   Centrul National de Management Programe - Biotech    

  Contractor:   University of Agricultural Sciences ad Veterinary Medicine Cluj-Napoca    

  Research Manager: Petrescu-Mag Ioan Valentin  

  Title of the project:   Development of&nbsp;RAPD markers associated to low water-temperature resistance&nbsp;in&nbsp;  Poecilia reticulata   Peters, 1859    

  Number of the project: 140/2006  

  Acronym: RAPDTEMP  

  Parteners:   

    UAIC Iasi    

    USAMV B Timisoara    

    USAMV Iasi    

  Period of the contract: 15 September 2006 – 15 September 2008  

  Initial team of the project:   

  USAMV Cluj-Napoca  

  Petrescu-Mag Ioan Valentin; Manager   

  Bud Ioan; Researcher  

  Pamfil Doru; Researcher  

  Grigoras Mircea Adrian; Asistant Manager   

  Vlaic Augustin; Researcher  

  Petrescu R. Malina; Researcher  

  Cosier Viorica; Researcher  

  Pop Rodica; Researcher  

  Georgescu Bogdan; Researcher  

  Ladoşi Daniela; Researcher  

  Boaru Anca; Researcher  

  Bâlteanu Valentin; Researcher  

  Raica Paul; Researcher  

  Dombi Istvan; Researcher  

  Vladau Vlad Viorel; Researcher  

  Pop Sorin; Researcher  

  Reka Stefan; Researcher  

  Petrescu Crina; Researcher  

  Botha Miklos; Researcher  

  Coroian Cristian; Researcher  

  Husovschi Adriana Maria; Economist  

  Florea Mariana; Economist  

  Molnar Florin; Student  

  Sabados Lavinia; Student  

  Hărşan Radu; Student  

  Maxim Elisabeta; Tehnician  

  UAIC Iasi  

  Gorgan Lucian; Group Leader  

  Cîmpeanu Mirela Mihaela; Researcher  

  Gherasim Raluca Sorina; Student  

  Buburuzan Laura; Student  

  USAMV B Timisoara  

  Bura Marian; Group Leader  

  Grozea Adrian; Researcher  

  Dumitrescu Gabi; Researcher  

  Patruica Silvia; Researcher   

  Vintilă Teodor; Researcher  

  Bănăţean-Dunea Ioan; Researcher  

  Muscalu-Nagy Radu; Researcher  

  Muscalu-Nagy Cristina; Researcher  

  Cozma Liliana; Tehnician  

  Korbuly Bogdan; Tehnician  

  Chiriţoiu Adrian; Tehnician  

  Ciobotaru Emilia; Economist  

  Popescu Ioan; Database Administrator  

  USAMV Iasi  

  Păsărin Benone; Group Leader  

  Stan Traian; Researcher  

  Gâlcă Ioan; Researcher   

  Creangă Steofil; Researcher   

  Usturoi Marius; Researcher  

  Confederat Margareta; Researcher  

  Marciuc Vasile; Researcher  

  Macovei Valerica; Researcher   

  Hoha Gabriel; Researcher  

  Diac Alexandrina; Researcher   

  Gruber Adrian; Researcher  

  Stănescu Roxana; Researcher  

  Radu Rusu Răzvan; Researcher  
]]></description><pubDate>Sat, 01 Nov 2008 16:03:26 +0000</pubDate><link>http://www.bioflux.com.ro/ceex-140/details-regarding-ceex-1402006/</link><guid>http://www.bioflux.com.ro/ceex-140/details-regarding-ceex-1402006/</guid></item><item><title><![CDATA[CEEX]]></title><description><![CDATA[  There are, in aquaria, coldwater fish species and tropical fishes, the guppy being one of the latter (Bud 2002). However, the guppy has the ability to survive in cooler waters that would certainly kill most tropical fishes (Mag &amp; Bud 2006; Mag et al 2006; Păsărin et al 2007). In their paper “Detection of a low temperature-resistant gene in guppy (  Poecilia reticulata  ), with reference to sex-linked inheritance”, Fujio et al (1990) reported an X-linked gene, responsible of low water-temperature resistance in guppy. Sixteen years later, in the paper: “Effect of temperature on sex ratio in guppy   Poecil    ia reticulata   (Peters 1860)”, Karayϋcel et al (2006) emitted the hypothesis according to which the same gene could be involved in high water-temperature resistance of guppy. In that moment, the above mentioned gene became a very important one, especially for guppyculture and generally for aquaculture, because of extrapolation possibility of these studies to many cases of economical and commercial important species.   

  In a CEEX-Biotech project (Research of Excelence Program, no 140/2006) our team tested a number of 150 random primers (Biosearch Technologies Inc.) in order to identify RAPD markers which could be associated to low temperature resistance in guppy. However, 71 random primers have not amplified DNA at all, 35 primers have not generated DNA polymorphisms, and 44 of them generated (more or less) polymorphisms. Only one random primer (no 77) was associated with cold tolerance (5'd(CCAACGACCA)3'; see Figure 1) in the Red Blond variety.  

  Figure 1.&nbsp;  http://www.bioflux.com.ro/docs/vol1/2008.1.99-110.pdf  &nbsp; Polymorphism, induced by the primer 77, associated with cold tolerance in the Red Blond guppy. The supplementary DNA fragment of 878 bp is associated to coldwater resistance (11-20), and the supplementary fragment of 839 bp is associated to coldwater sensitivity (1-10).  

  UPGMA (Unweighted Pair Group Method with Arithmetic Mean) was used to illustrate the two distinct fish clades: coldwater resistant group and coldwater sensitive group (see Figure 2). However, using the same primer (77), our repeated RAPD analyses failed to discriminate between coldwater resistant and sensitive individuals in other guppy strains. The primer was useless even for identification of coldwater resistant fishes from an F  B   generation whose father and both grandfathers were Red Blond males.  

  After the identification of coldwater resistant individuals, the next step forward of the research in the project CEEX 140/2006 was obtaining of a homozygote guppy strain for coldwater resistance gene, and this was possible due to the X-linkage of   Nigrocaudatus   II (  Ni  ) which was used as color marker gene. The breeding program of the research was based on a ♂Red Blond X ♀Half-Black Black cross, followed by a backcross of F  1   females with the initial Red Blond male.  

  One hundred of individuals, homozygote for the coldwater resistance gene, were further tested for resistance to heat. The results indicated a significant higher tolerance to heat of these ones compared to control lot. These data supported the hypothesis of Karayϋcel et al (2006) and indicated the common identity of coldwater resistance gene and heat tollerance gene. This X-linked gene (for resistance) seems to encode a protein with multiple role, a molecular chaperone, a protein formally included in HSPs group. We concluded at least some of the HSPs were involved in both coldwater resistance and heat tollerance, modulating animal metabolism under different environmental conditions.&nbsp;   

  Figure 2.   http://www.bioflux.com.ro/docs/vol1/2008.1.99-110.pdf  &nbsp; The two phenotypic distinct groups illustrated by UPGMA (when genomic DNA was amplified with the primer no 77).&nbsp;&nbsp;&nbsp;   

    References    

  Bud I., 2002 [Aquariophilia]. Academicpres, Cluj-Napoca, Romania. [in Romanian]  

  Fujio Y., Nakajima M., Nagahama Y., 1990 Detection of a low temperature-resistant gene in the guppy (  Poecilia reticulata  ), with reference to sex-linked inheritance. The Japanese Journal of Genetics 65(4):201-207.   

  Karayücel I., Ak O., Karayücel S., 2006 Effect of temperature on sex ratio in guppy   Poecilia reticulata   (Peters 1860) Aquaculture Research 37:139–150.  

  Mag I. V., Bud I., 2006 Environmental factors’ impact upon sex-ratio in guppies (  Poecilia reticulata   Peters, 1859). In: 41st Croatian and 1st International Symposium on Agriculture - Opatija, pp. 523-524, Osijek, Croatia.&nbsp;   

  Mag I. V., Bud I., Petrescu R. M., 2006 Sex-dependent mortality in the guppyfish. Acta Ichtiologica Romanica 1:153-164.  

  Păsărin B., Gorgan L., Bura M., Botha M., Petrescu-Mag I. V., 2007 The invasive potential of the exotic guppyfish (  Poecilia reticulata   Peters 1859) in temperate zone. Studia Universitatis Babes-Bolyai, Ambientum 1-2:179-187.  
]]></description><pubDate>Fri, 31 Oct 2008 09:25:28 +0000</pubDate><link>http://www.bioflux.com.ro/ceex-140/ceex/</link><guid>http://www.bioflux.com.ro/ceex-140/ceex/</guid></item></channel></rss>
